Study on acute toxicity of boiled Antarctic Krill and shrimp powder
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摘要: 目的:通过急性毒性实验,初步了解南极磷虾蒸煮的整虾和虾粉的安全性。方法:24h内喂食由南极磷虾和虾粉制成的受试物样品,对照组喂食SPF鼠粮。连续观察7d以及相关记录。结果:煮虾和虾粉制作的样品水分含量分别为14.14%和15.47%,灰分含量分别为12.54%和10.93%,样品脂肪含量为15.95%和2.56%,干重氟含量分别为1399.84mg/kg和186.77mg/kg;7d观察,小鼠无死亡均属正常情况,实验组小鼠体重和对照组无明显差异;鼠粮、煮虾和虾粉的小鼠原样最大给样量分别是290.89、599.04、62.71g/kg,折算成70kg成人剂量分别为32.73、64.22、7.18g/kg;煮虾组的肝脏系数与对照组有极显著差异(p<0.01),其他均无显著差异。结论:小鼠对南极磷虾煮虾和虾粉的最大耐受量分别大于599.04g/kg和62.71g/kg,而南极磷虾煮虾可能对肝脏有损伤,其安全性需亚慢性实验进一步研究。Abstract: Objective:An acute toxicity test was used to obtain a preliminary understanding of the safety of boiled Antarctic Krill and shrimp powder. Methods:Lab mice were classified into three categories. Two tested ones were fed with samples made by boiled Antarctic Krill and shrimp powderrespectively and the controlled ones were fed by SPF mice food. Seven consecutive days of observation and relevant record were needed.Results:Moisture content of samples, made by boiled Antarctic Krill and shrimp powder, were 14.14% and 15.47%, respectively, ash content were 12.54% and 10.93%, respectively. Furthermore, crude fat content of dry samples were 15.59% and 2.56%, respectively. And fluoride level were 1399.84mg/kg and 186.77mg/kg, respectively.After seven consecutive days of observation, there were no death cases. In fact, all the mice were normal. To conclude, there was nosignificant difference between the tested and controlled mice. The original maximum given dose of SPF mice food, boiled Antarctic Krill and shrimp powder were 290.89, 599.04g/kg and 62.71g/kg, respectively. The converted adult doses were 32.73, 64.22g/kg and 7.18g/kg, respectively. There was highly significant difference (p <0.01) between liver coefficient of tested and controlled mice, and There was no significant difference between others. Conclusion:The maximum tolerance dose of boiled Antarctic Krill and shrimp powder were above 599.04g/kg and 62.71g/kg, respectively. The safety of boiled Antarctic Krill needs further subchronicresearch as it may be harmful to liver.
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Keywords:
- Antarctic Krill /
- acute toxicity test /
- maximum given dose /
- maximum tolerance dose
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[1] 孙松.南极磷虾[J].世界科技研究与发展, 2002, 24 (4) :57-60. [2] Tou J C, Jaczynski J, Chen Y C.Krill for human consumption:Nutritional value and potential health benefits[J].Nutrition Reviews, 2007, 65 (2) :63-77.
[3] Yoshitomi B.Utilization of antarctic krill for food and feed[J].Food Chemistry, 2004, 43 (2) :45-49.
[4] 孙雷, 周德庆, 盛晓风.南极磷虾营养评价与安全性研究[J].海洋水产研究, 2008, 29 (6) :57-64. [5] GB/T 15193.1-2003, 食品安全性毒理学评价程序[S]. [6] GB/T 21757-2008, 化学品急性经口毒性实验急性毒性分类法[S]. [7] 张铣, 刘毓谷.毒理学[M].北京:北京医科大学中国协和医科大学联合出版社, 1997:233-234. [8] GB/T 15193.3-2003急性毒性实验[S]. [9] 陈守良.动物生理学[M].北京大学出版社, 2005. [10] 葛鲁娜, 刘肖帅, 武晓云, 等.食品与药品[J].2012, 14 (5) :172-174. [11] 潘建明, 张海生, 刘小涯.南大洋磷虾富氟机制I.氟的化学赋存形态研究[J].海洋学报, 2000, 22 (2) :58-64. [12] GB/T 15193.1-21-2003, 食品安全性毒理学评价程序和方法[S].
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