The influence of double-coating on the effectiveness of probiotic microcapsules
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摘要: 为了考察海藻酸钠二次包衣对两歧双歧杆菌F-35包埋效果的影响,本研究分别制备得到了海藻酸钠微胶囊（AL-M）、蛋白质微胶囊（WP-M）和海藻酸钠二次包衣的微胶囊（AL-CM）,并从三个方面（壁材降解速度、益生菌释放速度和益生菌存活情况）对这三种微胶囊在连续模拟胃肠液环境下的降解进行了比较。结果表明,海藻酸钠二次包衣能够显著降低胃蛋白酶对乳清蛋白的降解作用。相比于AL-M和WP-M,AL-CM会延缓两歧双歧杆菌F-35在模拟肠液中的释放速率,并且在连续的模拟胃肠道中经过二次包以后的微胶囊中对两歧双歧杆菌F-35保护效果最好,两歧双歧杆菌F-35最终的存活率高达51.0%。Abstract: In order to evaluate the influence of double-coating using alginate on the effectiveness of probiotic microcapsules, this study prepared three different microcapsules:alginate microcapsules, protein microcapsules, alginate-coated microcapsules, and compared these three microcapsules in three aspects, including:degradation rate of coating material, release rate of microencapslated probiotics and survival rate of microencapsulated probiotics. The results showed that the outer alginate membrane could significantly reduce the degradation role of pepsin on whey protein matrix. Beside, the coated microcapsules could delay the release rate of Bifidobacterium bifidum F-35 in simulated intestinal juice, but they provided the best protection for microencapsulated cells in sequential simulated gastrointestinal juice, the survival rate was up to 51.0%。
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Keywords:
- alginate /
- double-coating /
- protein /
- microcapsules /
- Bifidobacterium bifidum
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