Study on extraction, purification and antimicrobial activity of pomegranate peel tannins
-
摘要: 石榴皮中含有丰富的单宁类物质,具有良好的抑菌活性。以丙酮为提取溶剂,采用超声波辅助法对石榴皮单宁进行提取,并用Amberlite XAD-16大孔吸附树脂纯化,经高效液相色谱对其组分进行分析后,采用琼脂稀释法做最小抑菌浓度实验,结果表明:石榴皮提取物的主要成分为安石榴苷,纯化后质量浓度由12.3%提高到64.2%;石榴皮粗提物对金黄色葡萄球菌、单核细胞增生李斯特氏菌、沙门氏菌和大肠杆菌的最小抑菌浓度分别为0.312、10、0.625mg/mL和大于10mg/mL,纯化物对四种菌的最小抑菌浓度分别为0.156、5、0.625、10mg/mL。Abstract: Pomegranate peel is rich in tannins which have a good antibacterial activity. Pomegranate peel tannins were extracted by acetone in ultrasonic-assisted extraction method. After that they were purified by Amberlite XAD-16 macroporous resin. Then high performance liquid chromatography (HPLC) analysis was taken to determine the components and agar dilution method was adopted to test the minimal inhibitory concentration (MIC) . The results indicated that the concentration of punicalagin, the main component of pomegranate peel extract, increased from 12.3% to 64.2% after purification. The MICs of pomegranate peel crude extract on Staphylococcus aureus, Listeria monocytogenes, Salmonella and E.coli were 0.312, 10, 0.625mg/mL and larger than 10mg/mL, while the MICs of purified extract for these four kinds of bacteria were 0.156, 5, 0.625 and 10mg/mL respectively.
-
Keywords:
- pomegranate peel tannins /
- purification /
- HPLC /
- antimicrobial activity
-
[1] 何锦, 李勇.石榴化学成分及其保健功能的研究进展[J].粮油食品科技, 2012, 20 (2) :42-45. [2] 董周永, 郭松年, 赵国建, 等.石榴果皮提取物抑菌活性研究[J].西北植物学报, 2008, 28 (3) :582-587. [3] Tiwari B K, Valdramidis V P, O’Donnell C P, et al.Application of natural antimicrobials for food preservation[J].Journal of Agricultural and Food Chemistry, 2009, 57 (14) :5987-6000.
[4] 乔树华.石榴皮抑菌活性的初步研究[D].北京:中国农业科学院, 2009. [5] Kanatt S R, Chander R, Sharma A.Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products[J].International Journal of Food Science&Technology, 2010, 45 (2) :216-222.
[6] Al-Zoreky N S.Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels[J].International Journal of Food Microbiology, 2009, 134 (3) :244-248.
[7] Hayrapetyan H, Hazeleger W C, Beumer R R.Inhibition of Listeria monocytogenes by pomegranate (Punica granatum) peel extract in meat pate at different temperatures[J].Food Control, 2012, 23 (1) :66-72.
[8] 李巨秀, 张小宁, 李伟伟.不同品种石榴花色苷、总多酚含量及抗氧化活性比较研究[J].食品科学, 2011, 32 (23) :143-146. [9] 朱静, 陆晶晶, 袁其朋.大孔吸附树脂对石榴皮多酚的分离纯化[J].食品科技, 2010, 35 (1) :188-193. [10] Seeram N, Lee R, Hardy M L, et al.Rapid large scale purification of ellagitannins from pomegranate husk, a byproduct of the commercial juice industry[J].Separation and Purification Technology, 2005, 41 (1) :49-55.
[11] 赵艳红, 李建科, 李国荣.石榴皮多酚纯化及其抗氧化活性表征[J].食品科学, 2010, 31 (11) :31-37. [12] 张茜, 贾冬英, 姚开, 等.大孔吸附树脂纯化石榴皮多酚[J].精细化工, 2007, 24 (4) :345-349. [13] Aqil F, Munagala R, Vadhanam M V, et al.Anti-proliferative activity and protection against oxidative DNA damage by punicalagin isolated from pomegranate husk[J].Food Research International, 2012, 49 (1) :345-353.
[14] 焦中高, 刘杰超, 周红平, 等.枣果中酚类物质的高效液相色谱分析[J].食品与发酵工业, 2008, 34 (3) :133-136. [15] 曲文娟, 张渭洁, 徐敏瑶, 等.高效液相色谱测定石榴皮水提取物中4种多酚化合物的含量[J].分析测试学报, 2011, 30 (12) :1425-1429. [16] 赵海燕, 杨永红, 刘泰然, 等.高效液相色谱法同时测定保健食品中的安石榴甙和鞣花酸[J].中国食品卫生杂志, 2012, 24 (4) :333-335. [17] Zhou Honghao, Yuan Qipeng, Lu Jingjing.Preparative separation of punicalin from waste water of hydrolysed pomegranate husk by macroporous resin and preparative highperformance liquid chromatography[J].Food Chemistry, 2011, 126 (3) :1361-1365.
[18] Klancnik A, Piskemik S, Jergek B, et al.Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts[J].Journal of Microbiological Methods, 2010, 81 (2) :121-126.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
