Research progress in the enzymatic modification
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摘要: 酶修饰技术可以提高酶的稳定性、催化效率、活力和延长半衰期等性质,使其更好的应用于当今食品、医药等行业。本文重点综述了蛋白质交联修饰、小分子化学修饰、辅助因子修饰等七种分子化学修饰方法和分子定向化修饰、定点突变修饰等三种基因工程修饰方法以及它们在食品、医药和化工行业的应用,并对酶修饰技术的发展前景进行了展望。Abstract: Enzymatic modification could improve its stability, catalytic efficiency, biological activity, mission life, and etc., which promotes its application in food and medicine industries. This review covered seven chemical modification methods such as protein cross-linking modification, small-molecules chemical modification, and cofactor modification, and three genetic engineering methods such as molecular orientation modification and site-directed mutagenesis modification, as well as their applications in the food, pharmaceutical and chemical industries. Additionally, the prospects of enzymatic modification development also be highlighted.
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Keywords:
- enzymatic modification /
- chemical modification /
- genetic engineering /
- application
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