Experimental studies on the toxic effect of grape seed proanthocyanidin extract
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摘要: 目的:观察葡萄籽提取物原花青素的急性毒性和长期毒性,为其安全应用提供参考。方法:急性毒性实验,对小鼠灌服不同浓度的原花青素混悬液,采用Bliss法计算其LD50。长期毒性实验,将SD大鼠随机分为正常对照组和原花青素高、中、低剂量(428.57、214.29、42.86mg(/kg·d))组,连续灌胃给药26周,停药恢复4周,观察大鼠的一般行为、体重增长、食量消耗,进行血液学及血液生化学指征、尿常规检查、系统尸解及组织病理学诊断。结果:原花青素小鼠LD50为3451.9mg/kg。长期毒性实验中,与同期对照组比较,原花青素高、中、低3个剂量组动物行为活动、进食量等均未见异常;血液学指标、血液生化指标和尿液指标未发现与供试品有关的异常改变,病理组织学检查未发现与原花青素相关的异常改变。结论:在本实验条件下未观察到葡萄籽提取物原花青素有明显毒性反应。Abstract: Objective:To study the acute and long-term toxicity of grape seed proanthocyanidin extract (GSPE) , in order to provide safety experimental proof for clinical application. Methods:In the acute toxicity study, mice were intragastrically administered with GSPE in different concentrations, and calculate the median lethal dose (LD50) by Bliss method. In the long-term toxicity study, rats were randomly divided into four groups:blank control group, maximum (428.57mg/ (kg·d) ) , middle (214.29mg/ (kg·d) ) , minimum (42.86mg/ (kg·d) ) dose group of GSPE. After continuously took GSPE orally for 26 weeks, rats behavior, weight gain, food consumption, blood test, biochemistry test, urine test, systematic autopsy and histopathologic diagnosis were observed. These above indexes were inspected again 4 weeks after cease of feeding. Results:The oral acute toxicity study of GSPE in mice revealed that the LD50 was 3451.9mg/kg. The long-term toxicity study showed that no significant differences were observed in rat's behaviors, food intake and relative examination indexes among the 3 different dosage groups of GSPE, which compared with normal control group. There was no abnormal result of blood test, biochemistry test, urine test and histopathologic diagnosis in rats. Conclusion:These studies demonstrated that GSPE was safe and did not cause any detrimental effects in vivo under these conditions in this study.
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