Study on optimization of the extraction of total flavonoids from date seeds by response surface methodology
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摘要: 采用响应面法优化枣核中总黄酮的超声提取工艺。在单因素实验的基础上,采用响应面分析法优化超声提取工艺条件。结果表明,枣核中总黄酮的最佳超声提取工艺为:料液比1∶50(g/mL)、超声功率305W、提取温度60℃,该工艺条件下枣核中总黄酮提取率为7.03mg/g,与预测值的相对误差为0.43%,实验值与预测值相吻合。枣核总黄酮提取工艺经优化后提取率得到有效提高,可为枣核的再利用提供参考。Abstract: The experiment is carried to achieve the optimal conditions for ultrasonic extraction of total flavonoids from Chinese date seeds.Base on single-factor experiments, the extraction conditions of the total extraction yield of flavonoids in the date seeds were optimized by response surface analysis method.The results showed that optimum extraction process conditions were as follows:solid-liquid ratio 1 ∶50, ultrasonic power 305W, extraction temperature 60℃.The result of verification experiment (7.03mg/g) was coincided with predictive value.The extraction ratio of total flavonoids was effectively improved with the optimum extraction conditions, which could provide a reference for the reuse of date seeds.
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Keywords:
- date seeds /
- total flavonoids /
- extraction ratio /
- response surface analysis
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[1] 张志国.冬枣核类黄酮的提取工艺研究及其生物功能初探[D].西安:陕西师范大学, 2006. [2] 张娜, 杨保求, 王倩, 等.枣核黄酮类物质的抗氧化性研究[J].安徽农业科学, 2010, 35:20037-20039. [3] 梁单, 张保存.黄酮类化合物提取和分离方法研究进展[J].周口师范学院学报, 2007, 24 (5) :87-88. [4] 李巧玲.黄酮类化合物提取分离工艺的研究进展[J].山西食品工业, 2003 (4) :6-7. [5] 高晓娟, 马志刚, 董琳, 等.响应面法优化伏毛铁棒锤根部总生物碱提取工艺[J].中药材, 2010 (10) :1628-1632. [6] Yan Y, Yu C, Chen J, et al.Ultrasonic-assisted extraction optimized by response surface methodology, chemical composition and antioxidant activity of polysaccharides from Tremella mesenterica[J].Carbohydrate Polymers, 2011, 83 (1) :217-224.
[7] 张春兰, 张锐利, 熊素英, 等.超声波辅助提取红枣核中总黄酮的研究[J].安徽农业科学, 2010 (12) :6503-6505. [8] 张吉祥, 欧来良.正交实验法优化枣核黄酮的超声提取工艺研究[J].食品工业科技, 2012 (10) :280-283. [9] 张进棠, 姚本林, 刘伏煌, 等.银杏叶提取物中总黄酮的紫外分光光度测定[J].武汉化工学院学报, 1998 (1) :27-29. [10] 郭雪峰, 岳永德.黄酮类化合物的提取·分离纯化和含量测定方法的研究进展[J].安徽农业科学, 2007 (26) :8083-8086. [11] Yamazaki, Kushida N, Oguchi A, et al.Response Surface Design and Analyses[M].New York:Marcel Dekker Inc, 1987:149-205.
[12] 陈志伟, 陈坤, 胡银川, 等.响应面法对苦丁茶总黄酮提取工艺的优化[J].西南大学学报:自然科学版, 2012 (5) :119-125. [13] 王梅英, 陈慧斌, 陈军.响应面法优化超声波辅助提取岩茶总黄酮工艺研究究[J].食品工业科技, 2011, 32 (12) :327-330.
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