Promoter P43 regulatory effect for subtilosin A synthesis by expressing key enzyme gene
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摘要: 为提高枯草芽孢杆菌168（Bacillus subtilis 168）细菌素subtilosin A的产量使其能够应用到食品防腐、医药卫生等行业。采用重叠延伸PCR将强启动子P43分别和subtilosin A合成酶基因簇中关键酶基因sbo-albA、albB-albC连接到一起,构建了高表达载体。采用改进的电击法将重组载体转化到Bacillus subtilis 168中,通过高效液相色谱（HPLC）检测,确定subtilosin A产量得到了提高,进一步通过抑菌实验证明其抑菌活性也获得了提高。该方法为提高subtilosin A产量,进而应用到生产实践中提供了理论参考。
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关键词:
- 启动子 /
- subtilosin A /
- 关键酶 /
- 调控
Abstract: For improving Bacillus subtilis 168 bacteriocin subtilosin A production and applying to food preservation, medical science and so on, P 43 was linked to key enzyme gene sbo-albA, albB-albC of subtilosin A operon using SOE-PCR.High expressing vector was constructed.This recombinant vector was transformed into Bacillus subtilis 168 by high osmolarity electroporation method.HPLC assay showed subtilosin A production was improved.Inhibited method also showed the improving.This method will provide theory reference of improving subtilosin A production and applying in productive practice.-
Keywords:
- promoter /
- subtilosin A /
- key enzyme /
- regulation
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