Analysis of Zijuan tea aroma components of fully automatic HS-SPME
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摘要: 采用全自动顶空固相微萃取（headspace solid-phase microextraction,HS-SPME）结合气相色谱-质谱（gas chromatography-mass spectrometry,GC-MS）分析云南特种紫娟晒青茶和紫娟烘青茶香气成分,并比较利用常规大叶种制成的晒青毛茶的香气成分的差异。结果表明,紫娟晒青茶和紫娟烘青茶香气成分较接近,但比大叶种茶丰富,且差异较大;紫娟晒青茶的香气成分主要是芳樟醇、二氢猕猴桃内酯、2,6-二甲基-3,7-辛二烯-2,6-二醇、咖啡因、6,10,14-三甲基-2-十五烷酮、β-紫罗酮、十六烷、植醇、香叶醇、芳樟醇氧化物、α-松油醇等;紫娟烘青茶的香气成分主要是植醇、咖啡因、芳樟醇、十六烷、芳樟醇氧化物、二氢猕猴桃内酯、十八烷、茉莉酮、苯甲酸叶醇酯、橙花叔醇、十七烷等;而大叶种晒青茶的香气成分主要是植醇、芳樟醇、咖啡因、β-紫罗酮、邻苯二甲酸二异丁酯、6,10,14-三甲基-2-十五烷酮、十六烷酸、邻苯二甲酸二丁酯、香叶基丙酮等。
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关键词:
- 紫娟茶 /
- 大叶种茶 /
- 全自动顶空固相微萃取（HS-SPME） /
- 香气成分 /
- 气相色谱-质谱（GC-MS）
Abstract: The aromatic components of Yunnan specific Zijuan sun-dry green tea and Zijuan basket-fired green tea were extracted by fully automated headspace solid-phase microextraction (HS-SPME) , and were identified by gas chromatography-mass spectrometry (GC-MS) . Then comparison difference with the regular Daye sundry green tea was made. Results showed that the aromatic components of Zijuan sun-dry green tea and Zijuan basket-fired green tea were very close, but more abundant than that of Daye sun-dry green tea. The major aromatic components in Zijuan sun-dry green tea were linalool, dihydroactinidiolide, 3, 7-octadiene-2, 6-diol2, 6-dimethyl, caffeine, 6, 10, 14-trimethyl-2-Pentadecanone, beta-ionone, hexadecane, phytol, geraniol, linalool oxide, α-terpineol, the major aromatic components in Zijuan basket-fired green tea were phytol, caffeine, linalool, hexadecane, linalool oxide, dihydroactinidiolide, octadecane, jasmone, benzoic acid cis-3-hexenyl ester, nerolidol, heptadecane, while the major aromatic components in Daye sun-dry green tea were phytol, linalool, caffeine, beta-ionone, diisobutyl phthalate, 6, 10, 14-trimethyl-2-Pentadecanone, hexadecylic acid, dibutyl phthalate, geranylacetone, etc. -
[1] 王秋萍, 龚加顺.“紫娟”普洱茶茶褐素对高脂饮食大鼠生长发育的影响[J].茶叶科学, 2012, 32 (1) :87-94. [2] 陈宗懋.中国茶叶大辞典[M].北京:中国轻工业出版社, 2000:105-109. [3] 包云秀, 夏丽飞, 李友勇, 等.茶树新品种‘紫娟’[J].园艺学报, 2008, 35 (6) :934. [4] Jiang L H, Shen X J, Shoji T, et al.Characterization and activity of anthocyanins in Zijuan tea (Camellia sinensis var.kitamura) [J].Journal of Agricultural and Food Chemistry, 2013, 61 (13) :3306-3310.
[5] Zhao Y, Xu Y, Li J, et al.Profile of volatile compounds in 11Brandies by headspace solid-phase microextraction followed by gas chromatography-mass spectrometry[J].Journal of Food Science, 2009, 74 (2) :C90-C99.
[6] Xiao Z B, Dai S P, Niu Y W, et al.Discrimination of Chinese vinegars based on headspace solid-phase microextraction-gas chromatography mass spectrometry of volatile compounds and multivariate analysis[J].Journal of Food Science, 2011, 76 (8) :C1125-C1135.
[7] Stoppacher N, Kluger B, Zeilinger S, et al.Identification and profiling of volatile metabolites of the biocontrol fungus Trichoderma atroviride by HS-SPME-GC-MS[J].Journal of Microbiological Methods, 2010, 81 (2) :187-193.
[8] Musshoff F, Junker H P, Lachenmeier D W, et al.Fully automated determination of amphetamines and synthetic designer drugs in hair samples using headspace solid-phase microextraction and gas chromatography-mass spectrometry[J].Journal of Chromatographic Science, 2002, 40 (6) :359-364.
[9] Musteata M L, Musteata F M.Analytical methods used in conjunction with solid-phase microextraction:a review of recent bioanalytical applications[J].Bioanalysis, 2009 (6) :1081-1102.
[10] 梁名志, 夏涛.特种紫茶降压活性物质初探[J].云南农业大学学报, 2003, 18 (4) :378-381. [11] Gong J S, Zhang Q, Peng C X, et al.Curie-point pyrolysisgas chromatography-mass spectroscopic analysis of theabrownins from fermented Zijuan tea[J].Journal of Analytical and Applied Pyrolysis, 2012, 97:171-180.
[12] 夏丽飞, 陈林波, 蔡丽, 等.特种紫娟茶与大叶茶香气成分比较研究[J].西南农业学报, 2010, 23 (5) :1424-1428. [13] 王秋萍, 龚加顺, 张蕙.云南“紫娟”晒青绿茶和大叶晒青绿茶的化学成分比较研究[J].中国食品学报, 2012, 12 (1) :213-220. [14] 张超, 卢艳, 李翼新, 等.茶叶香气成分以及香气形成的机理研究进展[J].福建茶叶, 2005 (3) :17-19. [15] 杨明容, 马燕, 杨四润, 等.气质联用分析云南老树茶香气成分[J].中国农学通报, 2012, 28 (24) :261-269. [16] 钟秋生, 吕海鹏, 林智, 等.东方美人茶和铁观音香气成分的比较研究[J].食品科学, 2009, 30 (8) :182-186. [17] 孙宝国, 孙金沅.邻苯二甲酸酯类化合物的天然存在及安全性问题的探讨[J].中国食品学报, 2011, 11 (8) :11-18. [18] 王峰, 曹福亮, 张洪娟.SPME法与SDE法测定雨花茶香气成分比较[J].林业科技开发, 2010, 24 (3) :123-125. [19] 宛晓春.茶叶生物化学[M].北京:中国农业出版社, 2003:427-451. [20] 林翔云.香料香精辞典[M].北京:化学工业出版社, 2007:15-466. [21] 杨兴荣, 包云秀, 黄玫.云南稀有茶树品种“紫娟”的植物学特性和品质特征[J].茶叶, 2009, 35 (1) :17-18.
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