培养条件对单增李斯特菌生长的影响
详细信息Study the effect of cultural condition on growth state of Listeria monocytogenes
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摘要: 以纯度为99.97%的单增李斯特菌为研究对象,通过测定菌株在各培养条件下菌液的OD600值,分析了不同的NaCl浓度、pH、温度及NaCl浓度为3%～8%(梯度为1%),pH为6～9(梯度为1),温度为0～40℃(梯度为10℃)的交互作用条件下单增李斯特菌的生长状况。结果表明:菌株的对数增长期为8～14h,稳定期为14～18h,18h以后进入衰亡期;菌株在NaCl浓度为0.5%～3.5%范围内生长良好,其最适生长pH为7.5,最适生长温度为37℃。各因素的交互作用通过SPSS软件进行方差分析,得到其p<0.01,即各因素的交互效应极显著。Abstract: This study was based on Listeria monocytogenes of 99.97% purity, and determined the growth curve of strain before changing medium conditions, and then culture conditions had been adjusted.By measuring the OD600 value of bacteria liquid under different cultural condition, the growth status of Listeria monocytogenes under different NaCl concentration, pH value, temperature and their interactions under NaCl concentration 3% ～8% ( one for per gradient) , pH 6～9 ( one for per gradient) , temperature 0～40℃ ( 10℃ for per gradient) had been analyzed. The results showed that the logarithmic phase of this strain was 8 ～14h, the stationary phase was 14 ～18h, and it entered into decline phase after 18h. It could grow well in range of 0.5% ～ 3.5% concentration of NaCl and its optimum growth pH value was 7.5, and its optimum growth temperature was 37℃. The interaction effect of each factor had been analyzed by means of SPSS software, and variance analyses had been carried out, and results showed that the p < 0.01, and that means interaction effects were quite notable.
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