猫棒束孢真菌多糖提取工艺研究
详细信息Extraction technology of polysaccharide from Isaria felina
-
摘要: 为优化猫棒束孢真菌多糖提取工艺,采用液体发酵法培养猫棒束孢菌丝体,采用水溶醇沉法提取猫棒束孢真菌多糖,采用苯酚-硫酸比色法测定多糖含量,通过单因素实验和L9（33）正交实验,确定猫棒束孢真菌多糖的最佳提取工艺条件为:水料比6:1,提取液浓缩至原料重量的2倍,加入浓缩液4倍的无水乙醇,在此条件下多糖的提取率为16.24%。Abstract: Isaria felina was a new strain of Cordyceps sinensis that had been separated from the fructification of natural Cordyceps sinensis. The purpose of this article was to study the optimum extraction technology of polysaccharide from Isaria felina. The method of liquid fermentation was used to culture Isaria felina, the method of water extraction and alcohol precipitate was used to isolate polysaccharide from the mycelium of Isaria felina, and the method of phenol-sulfuric acid was used to determine the content of polysaccharide. Through the single factor and orthogonal test, result showed that the optimum extraction conditions were as follows:the ratio of water and mycelium powder was 6:1, the extract was concentrated to twice the weight of mycelium powder and the ethanol ratio was four times the volume of the concentrated extract. Under this condition, the extraction rate of polysaccharides was 16. 24%.
-
[1] Hikino H, Konno C, Mirin Y, et al.Isolation and Hypoglycemic Activity of Ganoderans A and B, Glycans of Ganoderma lucidum Fruit Bodies1[J].Planta Med, 1985, 51 (4) :339-340.
[2] 胡晶, 吴宏.当归多糖对小鼠外周血造血干细胞动员作用的研究[J].中草药, 2006, 37 (12) :1835-1838. [3] Jiang J H, Slivova V, Harvey K, et al.Ganoderma lucidum suppress growth of breast cancer cells through the inhibition of A kt/N F kappa B signaling[J].Nutr Cancer, 2004, 49 (2) :209-216.
[4] 邓宇, 窦晓兵, 史亦谦, 等.黄芪多糖定向诱生脐血来源树突状细胞及其对T细胞增殖作用的研究[J].中国免疫学杂志, 2007, 23 (6) :539-544. [5] 赵镕, 高永翔.黄芪多糖的免疫调节作用研究进展[J].中医临床研究, 2012, 4 (5) :4-6. [6] 高丽君, 王汉忠, 崔健华, 等.苯酚-硫酸法测定白首乌中多糖含量[J].山东农业大学学报, 2004, 35 (2) :295-297.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
