五倍子抗蕃茄晚疫病原菌有效成分提取工艺研究
详细信息Extraction of anti-tomato late blight from Galla chinensis and study on the extracting technology
-
摘要: 对不同提取条件下五倍子提取液抗蕃茄晚疫病原菌作用进行比较,优选出最佳提取工艺,并测定了其最小抑菌浓度、最小杀菌浓度。结果最佳提取工艺为:水为溶剂,在80℃浸提3h,料液比1:25,提取2次。五倍子提取液对蕃茄晚疫病原菌最小抑菌浓度为15.7mg/mL,最小杀菌浓度为31.3mg/mL。在此条件下五倍子对番茄晚疫病菌的抑菌直径为33.2mm。Abstract: This paper studied the antifungal activity components extracted from Galla chinensis agnist tomato late blight, with the minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) were measured . The optimal exraction parame-ters for Galla chinensis were obtained as followed : extraction temperature 80℃, extraction time 3h, extraction times 2 and the ratio of herb to water was 1∶25. The minimum inhibitory concentration (MIC) was 15.7mg/mL, MFC was 31.3mg/mL. The inhibition zone for tomato late blight reached 33.2mm under circvmstances mentioned above.
-
Keywords:
- Galla chinensis;tomato late blight;extract;MIC; /
-
[1] 李秀萍, 李春远, 梁桂荣, 等.五倍子的研究概况[J].中医药学报, 2002, 30 (3) :70-82. [2] 姜创, 吴相, 刘军海.超声波辅助法提取五倍子中单宁酸的工艺研究[J].食品与发酵科技, 2009, 45 (2) :11-13. [3] 帅益武, 尤玉如, 袁海娜.五倍子中鞣质的提取、分离纯化研究[J].食品科技, 2007 (6) :125-128. [4] Hebrert Kolodziej, Oliver Kyaser, Oliver A Radtke, et al.Pharmacological profile of extracts of pelargonium sidoides and their constituents[J].Phytomedicine Suttgart, 2003 (10) :18.
[5] 李春远, 丁唯嘉, 梁桂荣.五倍子化学成分的研究[J].中草药学报, 2008, 39 (8) :1129-1132. [6] 何平高.蕃茄晚疫病发生规律及防治方法[J].四川农业科技, 2005 (4) :32. [7] William E Fry, Stephen B, Goodwin.Re-emergence of potato and tomato late blight in the united states[J].Plant Disease, 1995, 79 (8) :539-540.
[8] R D Peters, H W Patt, R Hall.Hypotheses for the interregional the interregional movement of new genotypes of phytophthora infestans in Canada[J].Cana J Plant Plant Pathol, 1999, 21:132-136.
[9] 周红晞.高等植物源农药[J].农药译丛, 1994, 16 (2) :1-6. [10] 李正英, 张保军, 陈忠军.杂交弧瓜中抑菌物质提取条件的优选[J].食品科学, 2007, 28 (8) :209-212. [11] 王新伟, 杜会云, 宋玉函, 等.牛至油、香芹酚、柠檬醛和肉桂醛抗真菌研究[J].食品科技, 2011, 36 (2) :193-202. [12] 景维本.相似相溶原理浅析[J].教学与管理, 1986:75-76. [13] 陈庆敏.凤尾兰提取物抑菌活性研究[D].泰安:山东农业大学, 2007.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
