藤茶黄酮含量及其体外抗脂质过氧化能力研究
详细信息Study on content and anti-lipoperoxidation activity in vitro of flavonoids extracted from Ampelopsis grossedentata
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摘要: 分别采用超声波辅助热水法、超声波辅助乙醇法、乙醇浸提法、酶解法的最佳提取工艺提取藤茶黄酮,以AlCl3显色法测定黄酮含量;选择最佳方法测定不同时期、不同部位藤茶的黄酮含量。对比研究了藤茶叶和维生素体外抗脂质过氧化作用。结果表明:不同时期、不同部位藤茶的黄酮含量具有明显差别,叶中黄酮含量明显高于茎,高达41.25%;在555μg/mL内藤茶叶的抗脂质过氧化作用明显强于维生素C,并且在实验浓度范围内呈剂量关系。Abstract: The flavonoids from Ampelopsis grossedentata were extracted respectively by the best extraction technology of ultrasonic auxiliary hot water method, ultrasonic auxiliary ethanol method, ethanol leaching method and enzyme method.The content of flavonoids were determined by spectrophotometry using chromogenic reaction of AlCl3.Choosing the ultrasonic auxiliary ethanol method from which the content of flavonoids was the highest to determine the content of different periods and different parts.The anti-lipoperoxidation activity in vitro on the liver homogenate of mouse which was made by the flavonoids in Ampelopsis grossedentata leaves and vitamin C was studied contrastively.The result showed:the content of flavonoids in different periods and different parts had evident difference.Namely, the content in leaves was much higher than in stem (41.25%) ;the anti-lipoperoxidation effect of the flavonoids in Ampelopsis grossedentata leaves was evident stronger than vitamin C and it showed did relationship within the experimental concentration range.
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