竹叶黄酮对CCl4所致小鼠肝细胞损伤的保护作用
详细信息Protective effects of bamboo leaf flavonoids on damage of hepatocytes injured by CCl4
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摘要: 以研究竹叶黄酮对CCl4所致小鼠肝细胞损伤的保护作用为目的,通过建立CCl4损伤肝细胞模型,观察不同剂量的竹叶黄酮对谷丙转氨酶（ALT）、谷草转氨酶（AST）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）、肝糖元含量的影响,并观察空白对照组、模型组及各剂量组肝组织石蜡切片的病变情况。结果显示,与空白组相比,模型组ALT、AST、MDA显著升高（p<0.05）,肝糖元含量、GSH-Px和SOD活性明显下降（p<0.05）。与模型组比较,在各剂量竹叶黄酮的影响下,ALT、AST和MDA含量下降（p<0.05）,GSH-Px、SOD活性和肝糖元含量显著升高（p<0.05）,并呈剂量依赖性,肝组织病变程度明显低于模型组。本实验证明竹叶黄酮对CCl4所致小鼠肝细胞损伤有较好的保护作用。Abstract: To study on protective effects of bamboo leaf flavonoids (BLF) on damage of hepatocytes injured by CCl4, models of hepatocytes damage induced by CCl4 was established, the activity of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , glutathione peroxidase (GSH-Px) , superoxide dismurase (SOD) and the content of malondialdehyde (MDA) , liver glycogen was detected on the effect of different dosages groups of bamboo leaf flavonoids, and lesion level of liver tissues was observed through paraffin section in model group and different dosages groups. The results showed that compared with the normal control group, the lever of ALT, AST, MDA increased significantly in model group (p<0.05) , the content of liver glycogen and the activity of GSH-Px, SOD was decreased remarkably (p<0.05) . Compared with model group, on the effect of different dosages of BLF, the content of ALT, AST and MDA was decreased (p<0.05) , the level of GSH-Px, SOD and liver glycogen increased significantly (p<0.05) , and showed dose-dependence. Meanwhile, the lesion level of liver tissues was lower than model group obviously. It could be proved that bamboo leaf flavonoids have remarkable protective effect on hepatocytes damage injured by CCl4.
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