灵芝子实体超临界CO2萃取物与醇提物生物活性的比较
详细信息Comparison on bioactivities of supercritical CO2 fluid extracts and ethanol extracts from the fruit body of Ganoderma lucidum
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摘要: 采用超临界CO2萃取法和醇提法对灵芝子实体进行提取,利用体外抗肿瘤、抗神经细胞损伤和抗氧化模型比较了两种方法获得的萃取物生物活性的差异。在100μg/mL时,灵芝子实体超临界CO2萃取物（SFE-CO2E）对肿瘤细胞LNCaP、K562、HepG2增殖的抑制率分别为78.37%、87.09%、78.85%,均高于灵芝子实体醇提物（EtOHE）的60.65%、82.29%、66.15%;在体外抗神经细胞损伤模型中,在200μg/mL时SFE-CO2E对PC12的修复率为59.29%,优于EtOHE的17.77%;然而在清除羟基自由基、H2O2和DPPH自由基的实验中,在800μg/mL时,EtOHE的清除率分别为61.90%、67.33%、52.07%,均高于SFE-CO2E的29.24%、39.43%、17.05%。可见,超临界CO2萃取法和醇提法在提取灵芝子实体的不同的活性物质上各有优势。Abstract: The comparison on bioactivities of two sorts of extracts from the fruit body of Ganoderma lucidum obtained from supercritical CO2 fluid extraction (SFE-CO2) and ethanol extraction separately was investigated through applying a series of in vitro analytical methods including antitumor model, neurocyte anti-injury models and antioxidant model. The inhibition rate of supercritical CO2 fluid extracts (SFE-CO2E) against proliferation of tumor cell LNCaP, K562 and HepG2 was 78.37%, 87.09% and 78.85% respectively when inhibitory concentration reached 100μg/mL, which were higher than the inhibition effect of ethanol extracts (EtOHE) with 60.65%, 82.29% and 66.15% respectively. In vitro neurocyte anti-injury determination revealed that the repair effect of SFE-CO2E on cell PC12 (59.29%) was much more obvious compared with that of EtOHE (17.77%) at the concentration of 200μg/mL. The free radical scavenging experiments, however, demonstrated the scavenging activity of SFE-CO2E (29.24%, 39.43% and 17.05% respectively) was greatly lower than that of EtOHE (61.90%, 67.33% and 52.07% respectively) in analytical sequence of ·OH, H2O2 and DPPH· when effective concentration reached 800μg/mL. It was concluded that SFE-CO2 and ethanol extraction indicates various advantages on extracting different bioactive substances from the fruit body of Ganoderma lucidum.
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