青岛啤酒酵母与高浓酵母筛选高浓酿造酵母融合亲株100L发酵分析
详细信息Beer yeast and high concentrated yeast to screen of fusion strains with Qingdao for high concentration brewing yeast and 100L fermentation analysis
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摘要: 以青岛啤酒酵母T1、T2、T3和高浓酵母G4、G6为供试菌株,筛选生长良好的酵母,为筛选具有青岛啤酒风味的高浓酵母原生质体融合亲株做指导。比较了5株酵母菌100L发酵过程中酵母菌数量、双乙酰变化及待滤酒发酵度、酸度、α-氨基氮同化率等指标。结果表明:G4和G6酵母数量变化、双乙酰变化、发酵度和α-氨基氮同化率指标优于T1、T2和T3,青岛啤酒酵母中T1和T3除酵母凝聚性外其他指标都优于T2。因此,确定T1、T3和G4、G6为融合亲株。Abstract: In order to screen high consistency yeast parent strains of protoplast fusion, Qingdao beer yeast T1, T2 and T3 and high consistency yeast G4, G6 were used as tested strains.By comparing with the indexes in fermentation of 100L, such as yeast quantity, diacetyl change, fermentation degree, acidity, α-N assimilation rate, the result showed that the tested indexes of G4 and G6 were better than the indexes of T1, T2 and T3.Except for cohesion, the other indexes of T1 and T3 were better than those of T2.So, T1, T3 and G4, G6 were fusion parent strains.
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[1] 梅丛笑, 方元超, 王富荣.啤酒酵母的改良途径[J].食品工业科技, 2000, 21 (3) :70-73. [2] Venturini Filho W G, Cereda M P.Cassava starch hydrolysate as an adjunct in beer production:Chemical and sensory evaluation[J].Cienc Tecnol Aliment, 1998, 18 (2) :156-161.
[3] Javadekar V S, Sivaraman H, Gokhale D V.Industrial yeast strain improvement:construction of a highly floccu-lent yeast with a killer character by protoplast fusion[J].J Indus Microbiol, 1995, 15:94-102.
[4] 邓红梅.利用原生质体融合提高酿酒酵母赖氨酸含量的研究[J].贵州大学学报:自然科学版, 2002, 19 (3) :227-231. [5] 孙君社, 李雪, 李军席.原生质体融合构建耐高温酵母菌株[J].食品与发酵工业, 2002, 28 (5) :1-5. [6] 陈海昌, 唐屹, 张岭花, 等.原生质体融合技术提高啤酒酵母凝絮性的研究[J].微生物学通报, 1994, 21 (4) :213-217. [7] 周东坡, 平文祥, 孙剑秋, 等.通过灭活原生质体融合选育啤酒酵母新菌株[J].微生物学报, 1999, 39 (5) :454-460. [8] 蔡车国, 刘月英, 戴玉聪, 等.啤酒酵母融合株GR8发酵特性的初步研究[J].酿酒, 2005, 32 (4) :60-61. [9] 张宝国, 国晓秋, 王超, 等.啤酒酵母融合株QSB-XI6生产性实验[J].酿酒, 1998 (2) :29-30. [10] 全丽.啤酒酵母原生质体融合株GR8的中试研究[J].酿酒科技, 2005 (8) :69-73. [11] 刘月英, 王芬, 由媛, 等.啤酒酵母原生质体融合株HN3126的啤酒酿造中试研究[J].厦门大学学报:自然科学版, 2006, 45 (6) :855-858. [12] 中国食品发酵工业研究院, 酿酒工程技术研究发展部, 标准和信息技术研究发展部.啤酒工业国家标准与分析技术手册[M].北京:中国食品发酵工业研究院, 2001. [13] 母茜.高SOD, 低双乙酞啤酒酵母工程菌的构建[D].雅安:四川农业大学, 2008. [14] GuanDY.Handbook of Brewing Industry[M].Beijing:Ligt Industry Press, 1982:467-470.
[15] 刘杰璞.啤酒新产品的开发及风味研究[D].北京:北京化工大学, 2006. [16] 董新篁.定向培育啤酒酵母的技术[J].中国酿造, 2002, 123:24-35. [17] 江慧修, 张金玲, 周坚, 等.啤酒酿造用凝集性酵母融合育种的研究[J].微生物学报, 1993, 33 (1) :22-31.
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