枫香叶挥发油抗炎及其凝血作用以及化学成分的研究
详细信息Pharmacological activity and chemical composition of Liquidambar formosana leaves volatile oil
-
摘要: 研究了对枫香叶中的挥发油成分的抗炎活性以及凝血作用,并考察了其挥发油成分。实验通过建立BEAS-2B人肺上皮细胞炎症模型,加入地塞米松+TNF-α诱导细胞阳性对照组（Dex）、枫香叶挥发油+TNF-α诱导细胞实验组,检测IL-6蛋白的表达量,评价挥发油的抗炎效果。采用体外血浆复钙实验,考察枫香叶挥发油对大鼠血浆的促凝作用。结合气相与质谱联用技术（GC-MS）对枫香叶挥发油进行了结构鉴定和分析。证实了枫香叶挥发油确实具有良好的抗炎及凝血作用,鉴定了挥发油中存在26种化学成分,主要成分为:α-蒎烯、β-蒎烯、柠檬烯、β-石竹烯等,其中10种化学成分为首次报道。从而为枫香叶这种具有保健、食品、药材用途的天然产物的深入开发奠定了基础。Abstract: The Human bronchial epithelial (BEAS-2B) cells were pretreated with dexamethasone or essential oils prior to TNF-α stimulation.The anti-inflammatory activities were evaluated by the IL-6 release level.Lasma recalcification experiments were conducted to investigate the blood coagulation effect of the volatile oils in vitro.As a result, GC-MS method for analyzing the anti-inflammatory and blood coagulation activities of volatile oils from Liquidambar formosana was established.26 chemical compounds were identified by comparing with mass spectrum databases and literature data.Main ingredients included α-pinene, β-pinene, limonene, β-caryophyllene.A total of 10 compounds were reported for the first time.Thus the research laid the foundation for further development of Liquidambar formosana leaves which can be used in health care, food, medicine products.
-
[1] 翁玲玲, 蒋家谈, 张鼎画, 等.乡土树种枫香的研究现状与发展前景[J].福建林业科技, 2007, 34 (2) :184-185. [2] 广西壮族自治区革命委员会卫生局主编.广西本草选编 (上册) [M].南宁:广西人民出版社, 1974:1900-1901. [3] 郑毅, 张青.江西野生枫香活性成分提取及鞣质含量的研究[J].江西化工, 2004:99-102, 105-109. [4] 江蔚新, 赵玺.龙胆多糖的抗凝血作用研究[J].黑龙江医药, 2008, 21 (5) :31-32. [5] Kang Liu, Tianjiong Luo, Zhongai Zhang, et al.Modified Si-Miao-San extract inhibits inflammatory response and modulates insulin sensitivity in hepatocytes through an IKKβ/IRS-1/Akt-dependent pathway[J].Journal of Ethnopharmacology, 2011, 136:473-479.
[6] 赵宝明, 张志谦, 张书信, 等.广痛消泡沫气雾剂止血抗炎作用实验研究[J].中华中医药学刊, 2011, 29 (10) :2164-2166. [7] 吕小满, 胡艳芬.松塔化学成分及生物活性的研究进展[J].医学综述, 2010, 16 (7) :35-38. [8] 邹丽娟, 李杰.榄香烯抗癌作用与诱发肿瘤细胞凋亡的研究[J].大连医科大学学报, 1998, 20 (2) :87-90. [9] 夏文斌, 薛震, 李帅, 等.杜鹃蓝化学成分及肿瘤细胞毒活性研究[J].中国中药杂志, 2005, 30 (23) :1827-1829. [10] 李东霞, 张双全.鲨鱼软骨活性物质研究进展[J].河北中西医结合杂志, 1998, 7 (11) :1694-1696.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
