红酵母发酵废糖蜜产类胡萝卜素的研究
详细信息Study on the production of carotenoids by Rhodotorula glutinis in a molasses-based medium
-
摘要: 对红酵母利用糖蜜发酵产类胡萝卜素进行了研究,结果表明:对糖蜜的预处理的最佳酸为硫酸;利用经过酸处理后的糖蜜发酵,菌株的生物量为30.75g/L,单细胞色素含量为104μg/g,色素产量为26mg/L;最佳发酵时间为168h。使用糖蜜代替葡萄糖做廉价碳源对红酵母进行发酵,确定添加量为170g/L,色素产量最大值为葡萄糖发酵的0.97倍,生物量为1.04倍。Abstract: Carotenoid fermentation by Rhodotorula in a molasses-based medium was studied.The results showed that the optimal acid that had been used to pretreat molasses was sulfuric acid.When Rhodotorula was cultivated by acid-treated effluent from molasses, the maximal biomass of 30.75g/L was obtained, the maximal carotenoid content was 104μg/g, the carotenoid production was 26mg/L, the production of carotenoids by using molasses was 168h.On the basis of the optimal conditions, it was demonstrated that carotenoid yield and biomass fermented by Rhodotorula with molasses as cheap carbon source were 0.97 and 1.04 respectively times higher than that of those fermented with glucose.The optimum dosage of molasses was 170g/L.
-
Keywords:
- Rhodotorula;molasses;carotenoid;fermentation; /
-
[1] 王岁楼.红酵母产生类胡萝卜素的研究[J].中国粮油学报, 2001, 16 (5) :43-46. [2] 王伟霞, 李福后.红酵母RY-8菌株类胡萝卜素与SOD的纯化及部分性质[J].食品科技, 2009 (8) :31-33. [3] 张闯, 张玉苍, 何连芳.不同微生物生产类胡萝卜素的研究现状[J].食品研究与开发, 2011 (2) :11-12. [4] 陈欣.红酵母发酵产类胡萝卜素条件的优化[J].食品科学, 2009 (15) :49-50. [5] 赵颖, 罗璇, 钟晓凌, 等.响应面法优化产类胡萝卜素红酵母液体发酵培养基的研究[J].化学与生物工程, 2007 (12) :35-36. [6] 张闯, 何连芳, 张玉苍.红酵母生物合成类胡萝卜素的培养条件优化[J].中国酿造, 2010 (11) :32-34. [7] MALDONADE I R, RODRIGUEZ-AMAYA D B, SCAMPARINIA R P.Carotenoids of yeasts isolated from the Brazilian e-cosystem[J].Food Chemistry, 2008, 107 (1) :145-150.
[8] Frengova GI, Beshkova DM.Carotenoids from Rhodotorula and Phaffia:yeasts of biotechnological importance[J].Ind Microbiol Biotechnol, 2009, 36 (2) :163-180.
[9] Buzzini P, Innocenti M, Turchetti B, et al.Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus[J].Can J Microbiol, 2007, 53 (8) :1024-1031.
[10] BreierováE, Gregor T, MarováI, et al.Enhanced antioxidant formula based on a selenium-supplemented carotenoid-producing yeast biomass[J].Chem Biodivers, 2008, 5 (3) :440-446.
[11] 刘琦, 辛嘉英.红酵母及红酵母产类胡萝卜素研究进展[J].食品工业科技, 2010, 11 (3) :23-26. [12] 张颖鑫, 辛嘉英, 刘书娟, 等.金属离子对红酵母菌产类胡萝卜素影响的研究[J].食品工业科技, 2010, 31 (1) :10-11. [13] 韩梅, 葛向阳, 钱和, 等.锁掷酵母高产类胡萝卜素的发酵条件的研究[J].食品工业科技, 2011, 32 (12) :47-48. [14] Doucha J, Lívansky K, Kotrbácek V, et al.Production of Chlorella biomass enriched by selenium and its use in animal nutrition:a review[J].Appl Microbiol Biotechnol, 2009, 83 (6) :1001-1008.
[15] Bumbak F, Cook S, Zachleder V, et al.Best practices in heterotrophic high-cell-density microalgal processes:achievements, potential and possible limitations[J].Appl Microbiol Biotechnol, 2011, 91 (1) :31-46.
[16] TINOI J, RAKARIYATHAM N, DEMING R L.Simples opti-mization of carotenoid production by Rhodotorulag lutinis using hydrolyzed mung bean waste flour as substrate[J].Process Biochemistry, 2005, 40 (7) :2551-2557.
[17] 顾国贤.酿造酒工艺学[M].北京:中国轻工业出版社, 1999:323-325. [18] 梁慧星, 李朝霞, 陈欣.红酵母类胡萝卜素提取工艺及色素稳定性研究[J].湖北农业科学, 2011, 50 (4) :815-817. [19] 杨文, 吉春明.一种简单的胞壁破碎方法[J].微生物学通报, 1995, 22 (1) :58-59.
计量
- 文章访问数: 138
- HTML全文浏览量: 9
- PDF下载量: 282
下载:
