测定雌马酚的紫外法建立与应用
详细信息Establishment and application of equol determination by ultraviolet spectrophotometry method
-
摘要: 分别以雌马酚标准品和食用大豆异黄酮人的尿液为样品,建立一种测定雌马酚的紫外分析方法。考察方法的特性及测定结果可靠性,其结果为:检出限5×10-10g/mL,定量限15×10-10g/mL。应用于人尿液雌马酚的测定,精密度4.45%,回收率100.25%,结果可靠。新建立的紫外法达到了定量分析的要求,同时具有操作简单、快速、测定成本低的优点,适合大批量样品的测定。Abstract: A UV spectrophotometer used for determination of equol was established. Using equol standard and the urine equol from the tester who ate the soybean isoflavones for samples, the ultraviolet method character of determine equol was studied through UV spectrophotometer to determine the detection limit and quantitative limit. The result was as follows:the detection limit was 5 ×10-10g/mL, the quantitative limit was 15 ×10-10g/mL.Application for equol measurement of human urine, the precision was 4.45% and the average recovery rate was 100.25%, the result was reliable. The new UV method conforms to the quantitative analysis of the requirements was, it had advantages of simple, rapid, and high sensitivity. It was suitable for soybean isoflavone and equol established measuirement application.
-
Keywords:
- UV detection;equol;urine samp; /
-
[1] Kenneth D R, Setchell, Carlo Clerici.Equol:History, Chemistry and Formation[J].Nutr, 2010, 1355-1359.
[2] Hedlundte, Johanneswu, Millergj.Soy is isoflavone idequol modulates the growth of benign and malignant prostatic epithe lialcells in vitro[J].Prostate, 2003 (1) :682-781.
[3] Duncanam, Merz demlowbe, Xux, et al.Premeno pausal equol excretors show plasma hormone profiles associated with lowered.risk of breast cancer[J].Cancer Epidemiol Biomarkers Prev, 2000, 9 (6) :5812-5861.
[4] Hwangj, Wangj, Morazxzonip, et al.The phytoestrogenequol increases nitricoxide a vail ability by inhibiting superoxide production:an antioxidant mechanism for cell2mediated LDL modification[J].Free Radical Bio Med, 2003, 34 (10) :12712-12821.
[5] Akisonc, Skorhe, Fitzgiobbonsed, et al.Urinary equol excretionin relation to22hydroxy estrone and16A2hydroxy estrone concent rations:an observational study of young to middle2aged women soy2derived phyto estro gensin human breast tissue and biological fluids by high2perfor mance liquid chromato graphy[J].Chromatogr B, 2003, 784 (1) :1372-1441.
[6] Setchellkdr, Brownnm, Lydeking.The clini calim portance of the metabolite equol aclue to the effective eness of soy and its isoflavones[J].Nutr, 2002, 132 (12) :35772-35841.
[7] 高若梅, 刘鸿皋.检出限概念问题讨论———IUPAC及其他检出限定义的综合探讨和实验论证[J].分析化学, 1993, 21 (10) :1232-1236. [8] 鲁静, 付凌.质量分析方法验证中检出限和定量限测定方法探讨[J].中国药品标准, 2012, 13 (1) :33-35. [9] 李笑梅, 安静.大豆异黄酮降解液中雌马酚的纯化工艺[J].食品科学, 2010, 31 (14) :128-131. [10] 张连富, 丁霄霖.番茄红素简便测定方法的建立[J].食品发酵工业, 2001, 27 (3) :51-55. [11] 金迪.172名上海居民尿中大豆异黄酮及其代谢产物雌马酚的调查研究[D].上海:第二军医大学, 2009.
计量
- 文章访问数: 155
- HTML全文浏览量: 26
- PDF下载量: 156
下载:
