植物乳杆菌ZS2058生物转化共轭亚油酸反应条件的优化研究
详细信息Study on condition optimization of conjugated linoleic acid bioconversion by Lactobacillus plantarum ZS2058
-
摘要: 对本实验室从泡菜中筛选到的植物乳杆菌ZS2058完整细胞生物转化共轭亚油酸的反应条件进行了系统研究。在1mL磷酸钾缓冲液反应体系中,通过单因素实验和响应面分析,确定最合适的反应条件为:亚油酸底物浓度为0.8mg/mL,细胞浓度为4×1010cfu/mL,反应温度为37℃,缓冲液pH为6.7。在此反应条件下,cis9,trans11-CLA的浓度为374.24μg/mL,转化率高达46.78%,这对于实现共轭亚油酸的高效生产和研究其生理功能具有重要的现实意义和理论价值。Abstract: The converted condition of LA to CLA by using Lactobacillus plantarum ZS2058, which was screened from the Chinese traditional fermented vegetable, was studied.According to the production of CLA, bioconversion of CLA by resting cells of L.plantarum ZS2058 in 1mL potassium phosphate buffer system was optimized.Through the single factor experiment and response surface analysis, 374μg/mL of c9, t11-CLA was obtained under the reaction condition as follows:0.8mg/mL LA solution, 4×1010cfu/mL cell mass, optimal temperature 37℃ and the optimal pH value 6.7, and the conversion ratio of c9, t11-CLA was 46.78%, which was valuable for producing CLA efficiently and studying its physiological function.
-
[1] Jones E L, Shingfield K J, Kohen C, et al.Chemical, physical, and sensory properties of dairy products enriched with conjugated linoleic acid[J].Journal of Dairy Science, 2005, 88 (8) :2923-2937.
[2] Pariza M.Perspective on the safety and effectiveness of conjugated linoleic acid[J].American Journal of Clinical Nutrition, 2004, 79:1132S-1136S.
[3] Pariza M, Park Y, Cook M.The biologically active isomers of conjugated linoleic acid[J].Progress in Lipid Research, 2001, 40:283-298.
[4] Guo Z, Zhang G W, Sun Y.Preparstiong of conjugated linoleic acid and identification of its isomers[J].Chinese Journal of Chemical Engineering, 2003, 11 (2) :130-135.
[5] Lin T Y.Conjugated linoleic acid production by cells and enzyme extract of Lactobacillus delbrueckii ssp.bulgaricus with additions of different fatty acids[J].Food Chemistry, 2006, 94 (3) :437-441.
[6] 周倩, 刘佩, 李海霞, 等.微生物合成共轭亚油酸机理的研究进展[J].食品工业科技, 2011, 32 (11) :468-474. [7] 杨宏, 叶淑红, 王晗, 等.混菌发酵产共轭亚油酸条件的优化[J].食品工业科技, 2011, 32 (8) :220-222. [8] Ogawa J, Kishino S, Ando A, et al.Production of conjugated fatty acids by lactic acid bacteria[J].Journal of Bioscience and Bioengineering, 2006, 100 (4) :355-364.
[9] 钮晓燕, 陈卫, 张灏, 等.植物乳杆菌ZS2058在磷酸盐缓冲液体系中生物转化共轭亚油酸[J].微生物学报, 2007, 47 (2) :244-248. [10] 周凌华, 张灏, 陈卫, 等.生物合成共轭亚油酸菌种的筛选与鉴定[J].无锡轻工大学学报, 2004, 23 (5) :53-57. [11] 许庆炎, 陈海琴, 田丰伟, 等.植物乳杆菌ZS2058生物转化产物共轭亚油酸的分析方法的研究[J].食品与发酵工业, 2008, 34 (1) :110-114.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
